Note that charging is worse at high magnification, and that it builds
up over time on the portion of the specimen being scanned. Try moving to
a new spot to capture images quickly on the fresh area before charging builds
up, or give the specific desired spot a few minutes to "de-charge" with
the beam turned off or positioned elsewhere, then try again.
A) Use the Variable Pressure mode; set the pressure at some value
higher than presently in use, and watch the image while the vacuum gague
displays the current pressure. Note the value at which the charging is reduced
(image improves) and reset the pressure accordingly (remember, the lower the
pressure setting, the stronger the vacuum).
B) Decrease the accelerating voltage (2-5KV), and switch to SEM mode.
C) Decrease the probe current.
D) Decrease the aperture size.
E) Change the settings for the image capture button (click on the black triangle
above it); use a composite (e.g., 32 frames) in the TV setting instead of one slow scan.
F) Use the tilt control to increase the angle between the specimen and the beam.
Consider experimenting with graphite paint to increase conductivity between
specimen and stub during future preparation.
If the problem is persistent and the project is important, please ask for
help!