To Increase Depth of Field for Specimens That Are Not Flat:

Use SEM mode, with low voltage if necessary to reduce charging.

Decrease the aperture diameter.

Increase the working distance.

Use the rotation and/or tilt controls to get a better angle on the desired portion of the specimen.

Note that depth becomes more problematic as magnification increases.

Consider altering specimen position during future preparation (reduce the desired feature to one plane if possible via dissection, adhesive, etc.)


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